| Classification: | Biological Diagnostics |
|---|---|
| Type: | Real Time PCR System |
| Certification: | CE |
| Group: | All |
| Reaction Volume: | 10-50UL |
| Fluorescent Channels: | 5/6 |
| Customization: |
|---|
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As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications.
| Model | PCR-Q96-5 | PCR-Q96-6 |
| Sample capacity | 96 | |
| Reaction volume | 10-50 μl | |
| Thermal cycle technology | Peltier | |
| Temperature control system | ||
| Max. Heating/Cooling rate | 6.0° C/s | |
| Heating temperature range | 4 - 100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2ºC @60ºC , ±0.3ºC @95ºC | |
| Temperature gradient setting range | 30-100°C | |
| Temperature gradient difference setting range | 1 - 36°C | |
| Detection system | ||
| Excitation light source | 5 monochrome high efficiency LEDs | 6 monochrome high efficiency LEDs |
| Detection device | PMT | |
| Detection mode | Time-resolved signal separating technology | |
| Excitation/detection wavelength range | 455-650nm/510-715nm | |
| Fluorescent channels | 5 channels | 6 channels |
| Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ | |
| Sensitivity | Single copy gene | |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR | |
| Dynamic range | 10 orders of magnitude copies | |
Q: Can QPCR be used directly upon arrival? Does new machine need calibration after transporation ?
A: It need to be unlock first when arrive . There is video instruction. No need calibartion .
Q: What is the requirment of PC when install software ?
A: It need Win10 system PC. For new PC, it need to install a driver before install software. During the runing of software, PC screen need to keep on , not use dormant status.
Q: What is the difference between 4 channel and 6 channel model ?
A: PCR-RT-96-6 has six detection channels. PCR-RT-96-4 has five detection channels. They all have 2 duplicated for same dyes SYBR / FAM. PCR-RT-96-4 have one channel extra - Cy3/NED/TAMRA
Q: What is the difference between Standard and Fast mode for Melting curve?
A: Both model QPCR have 2 FAM/SYBR channel. For SYBR , in melting curve FAST Mode, the 2 FAM channel will be open at same time and detection time will be shorter than standard mode. But FAST mode cannot be used in Taqman probe mode.
Q: Can we adjust the voltage(Gain value )of PMT Setting of different channel ?
A: It cannot adjust the voltage of each channel , but we can adjust the light path with 3levels accordng to customer requirement to meet the demand of different signal intensity.
Q: What is the Scanning method?
A: From the top to scan each tube in sequence. The advantage is - avoid the crosstalk and get accurate fluorescence value.
Q: What is the door direction ?
A: QPCR door is at front side of machine.
Q: Does QPCR result need to be corrected by ROX ?
A: Not need
Q: What is the Detection device of QPCR? What light source and how many?
A: The detection device is PMT. The light source is LED. X6-6 X4-5
Q: What is the difference between PMT and CCD detection
A: PMT is highly sensitive signal detector. The detector scans the fluorescence signal of each well separately. It can effectively reduce fluorescent crosstalk. CCD is like a high-sensitivity camera.It collects fluorescence values of all well positions at once.It takes less time.
Q: What is the sample volume ?
A: 10-50ul
Q: What size tube can be used on QPCR ?
A: Recommend 100ul PCR tube or low profile 200ul tube. If use 200ul high profile tube , it will appear steam in the tube which may affect the test result. If must use 200ul high profile tube, suggest to add paraffin oil .
Q: It only has Second unit when we set the timer, can we have option of Minute ?
A: The length of gene is usually 200bp, time required as Seconds level. Only in RT test , Minute is needed . Will consider to add Minute unit in future.
Q: Can we edit program while QPCR run the program ?
A: Cannot edit .
Q: During PCR running , how to check the previous test result ?
A: Click 'File- Open in frame '
Q: What is the ordinate of the amplification curve currently showing?
A: In the latest version of the software, the original fluorescence value, Rn value and the normalized fluorescence value curve can be displayed.
Q: In the absolute quantitative test, can result analysis show the average value and deviation?
A: It need to tick out Replicate (in sample setting interface) when set Replicate sample and standard sample as the same value , then it appears average value during analysis , but cannot show the deviation value.
Q: Can user delete those unnormal results whe make the standard curve ?
A: The software does not support direct edit after test , user can output the CT value data and edit it in excel, then make the standard curve in excel.
Q: How to avoid the wrong setting or miss-selection of channels ?
A: Software doesnot support analysis after result, Suggest user to tick out all the channels during setting.
Q: How to adjust the threshold value ?
A: Select Spine method, threshold line will appear and can be adjusted
Q: What is the suitable value of Threshold line ?
A: For Linearity curve , put it at inflection point. For logistic curve, put it at straight line place
Q: The application result is normal why data cannot output ? Why the aplication curve cannot display when tick out the reaction tubes position ?
A: Check the Return at the right corner of Amplification interface, click Return then can operate.
Q: Can user define the color of amplification curve ?
A: CAN NOT
Q: How to adjust the start point of baseline after test end ?
A: The baseline has been automatically corrected , not need to adjust . The latest version of the software provide the option of baseline adjustment.
Q: How to solve the issue that signal value reach to upper limit at platform stage?
A: Can adjust the concentration of fluorescene marker or set the PMT detection as Low level .
Q: Can we use Molecular beacon to do melting curve ?
A: Hardware can support this test, but software doesnot have this analysis function, will be added in the next version software.
Q: Does QPCR have HRM analysis ?
A: The software is not supported currently
Q: What need to be attention when move the instrument ?
A: Before move the instrument , put the machine in 96 well protection plate and lock the machine. Refer to the Lock operation video.
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