| Customization: | Available |
|---|---|
| Reaction Volume: | 15-100UL |
| Fluorescent Channels: | 2 |
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Description
The product can be used for real-time detection in pasture forest farm, breeding farm and water source. It is used for rapid diagnosis of disaster and epidemic disease, inspection and quarantine in the field of food safety, and scientific research in biological laboratories.
Advantages
| Model | PCR-Q88-4 |
| Sample Capacity | 8×0.2ml (8 well) |
| Consumable | Clear 0.2 ml PCR tube /8-tube strips |
| Reaction Volume | 15-100μl |
| Temperature control
technology |
Marlow customized Peltier allow 1.000.000 cycles |
| Temperature Range | 0-100ºC(Resolution:0.1ºC) |
| Max. Ramp Rate | 7ºC/s |
| Temperature fluctuation | ±0.1ºC |
| Uniformity | ±0.25ºC |
| Accuracy | ±0.25ºC |
| Hot Lid Temperature | 30-110ºC (Adjustable, default 105ºC) |
| Temperature Control | Block/Tube |
| Excitation wavelength | 460-628nm |
| Emission wavelength | F1:520-540nm
F2:540-580nm F3:571-612nm F4:628-692nm |
| Factory Calibrated Dyes | F1: FAM/SYBR Green I
F2:HEX/VIC/JOE/TET F3: ROX F4: CY5 |
| Excitation | Long life LED |
| Detection | High sensitivity photoelectric detector |
| Dynamic Range | 1-1010 Copies |
| Sensitivity | 1 copy |
| Feature function | Quantitative/qualitative, melting curve, genotyping |
| Date Export Formats | Excel,cvs,txt |
| Printing | Report can be printed (optional USB thermal printer) |
| Communication | WIFI/USB2.0 |
| Dimension0(L×W×H) | 195×165×140 mm |
| Net Weight | 3Kg |
| Electricity | 220VAC,50Hz DC15V 225W |
Q: Can QPCR be used directly upon arrival? Does new machine need calibration after transporation ?
A: It need to be unlock first when arrive . There is video instruction. No need calibartion .
Q: What is the requirment of PC when install software ?
A: It need Win10 system PC. For new PC, it need to install a driver before install software. During the runing of software, PC screen need to keep on , not use dormant status.
Q: What is the difference between 4 channel and 6 channel model ?
A: PCR-RT-96-6 has six detection channels. PCR-RT-96-4 has five detection channels. They all have 2 duplicated for same dyes SYBR / FAM. PCR-RT-96-4 have one channel extra - Cy3/NED/TAMRA
Q: What is the difference between Standard and Fast mode for Melting curve?
A: Both model QPCR have 2 FAM/SYBR channel. For SYBR , in melting curve FAST Mode, the 2 FAM channel will be open at same time and detection time will be shorter than standard mode. But FAST mode cannot be used in Taqman probe mode.
Q: Can we adjust the voltage(Gain value )of PMT Setting of different channel ?
A: It cannot adjust the voltage of each channel , but we can adjust the light path with 3levels accordng to customer requirement to meet the demand of different signal intensity.
Q: What is the Scanning method?
A: From the top to scan each tube in sequence. The advantage is - avoid the crosstalk and get accurate fluorescence value.
Q: What is the door direction ?
A: QPCR door is at front side of machine.
Q: Does QPCR result need to be corrected by ROX ?
A: Not need
Q: What is the Detection device of QPCR? What light source and how many?
A: The detection device is PMT. The light source is LED. X6-6 X4-5
Q: What is the difference between PMT and CCD detection
A: PMT is highly sensitive signal detector. The detector scans the fluorescence signal of each well separately. It can effectively reduce fluorescent crosstalk. CCD is like a high-sensitivity camera.It collects fluorescence values of all well positions at once.It takes less time.
Q: What is the sample volume ?
A: 10-50ul
Q: What size tube can be used on QPCR ?
A: Recommend 100ul PCR tube or low profile 200ul tube. If use 200ul high profile tube , it will appear steam in the tube which may affect the test result. If must use 200ul high profile tube, suggest to add paraffin oil .
Q: It only has Second unit when we set the timer, can we have option of Minute ?
A: The length of gene is usually 200bp, time required as Seconds level. Only in RT test , Minute is needed . Will consider to add Minute unit in future.
Q: Can we edit program while QPCR run the program ?
A: Cannot edit .
Q: During PCR running , how to check the previous test result ?
A: Click 'File- Open in frame '
Q: What is the ordinate of the amplification curve currently showing?
A: In the latest version of the software, the original fluorescence value, Rn value and the normalized fluorescence value curve can be displayed.
Q: In the absolute quantitative test, can result analysis show the average value and deviation?
A: It need to tick out Replicate (in sample setting interface) when set Replicate sample and standard sample as the same value , then it appears average value during analysis , but cannot show the deviation value.
Q: Can user delete those unnormal results whe make the standard curve ?
A: The software does not support direct edit after test , user can output the CT value data and edit it in excel, then make the standard curve in excel.
Q: How to avoid the wrong setting or miss-selection of channels ?
A: Software doesnot support analysis after result, Suggest user to tick out all the channels during setting.
Q: How to adjust the threshold value ?
A: Select Spine method, threshold line will appear and can be adjusted
Q: What is the suitable value of Threshold line ?
A: For Linearity curve , put it at inflection point. For logistic curve, put it at straight line place
Q: The application result is normal why data cannot output ? Why the aplication curve cannot display when tick out the reaction tubes position ?
A: Check the Return at the right corner of Amplification interface, click Return then can operate.
Q: Can user define the color of amplification curve ?
A: CAN NOT
Q: How to adjust the start point of baseline after test end ?
A: The baseline has been automatically corrected , not need to adjust . The latest version of the software provide the option of baseline adjustment.
Q: How to solve the issue that signal value reach to upper limit at platform stage?
A: Can adjust the concentration of fluorescene marker or set the PMT detection as Low level .
Q: Can we use Molecular beacon to do melting curve ?
A: Hardware can support this test, but software doesnot have this analysis function, will be added in the next version software.
Q: Does QPCR have HRM analysis ?
A: The software is not supported currently
Q: What need to be attention when move the instrument ?
A: Before move the instrument , put the machine in 96 well protection plate and lock the machine. Refer to the Lock operation video.
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